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KMID : 0644120150280020000
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Journal of Oriental Gynecology
2015 Volume.28 No. 2 p.0 ~ p.0
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Effects of Citri Reticulatae Viride Pericarpium on the Apoptotic Cell Death in Breast Cancer Cells
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Kim Ji-Eun
Park Soo-Yeon
Choi Chang-Won
Kim Kyeong-Soo
Kim Kyeong-Ok
Wei Tung-Shuen
Yang Seung-Joung
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Abstract
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Objectives: In the theory of Korean medicine, Citri Reticulatae Viride Pericarpium (CRVP) can soothe the liver to break qi stagnation, eliminate mass and relieve dyspepsia. This study was carried out to investigate the effects of CRVP on the apoptotic cell death in breast cancer cells.
Methods: In the present experiment, the effects of CRVP on proliferation rates, type of cell death, cell cycle distribution, and intracellular oxidative stress were investigated using MDA-MB-231 cells in vitro. In addition, the effects on expression levels of caspase 3, caspase 9, Bax and Bcl-2 were also investigated.
Results: Treatment with CRVP decreased proliferation rates in a dose dependent manner. ID50 (50% inhibitory dosage) was 175.4 μg/ml. In the CRVP treated group, cell volumes showed smaller than non-treated normal. In addition, CRVP
increased percentage of apoptotic and sub G1 arrested cells respectively. 200 μg/ml of CRVP treatment increased intracellular ROS level significantly. Finaly the expression level of caspase 3 and Bax/Bcl-2 ratio were elevated by treatment with CRVP respectively.
Conclusions: These results suggest that CRVP can trigger intrinsic apoptotic pathway in MDA-MB-231 cells.
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KEYWORD
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Citri Reticulatae Viride Pericarpium (CRVP), MDA-MB-231 cells (breast cancer cells), intrinsic apoptotic pathway
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